What are the precautions for using vacuum blood collection tubes?

1. Selection of vacuum blood collection tubes and injection sequence

The corresponding test tube can be selected according to the tested items. The blood sequence is culture, normal, test tube with solid antioxidant, test tube with liquid antioxidant. The purpose of the order is to minimize the concentration and anti-anticoagulant in the analysis caused by the collection of the test tube.

1. Use the order of glass tubes: blood culture tube, non-coagulant clear another tube, sodium citrate anticoagulation tube test tube. Tube order: blood culture tubes (yellow anti, tart) tubes (blue anti) tubes (blue anti) acid wash tubes, hoses or unfilled tubes, tear tubes or tubes unfilled tubes (unfilled) tubes ( catheter), powered test tube (grey).

2. Blood collection site and posture

Infants and young children can take blood from the medial and lateral borders of the thumb or heel according to the method recommended by the World Health Organization, preferably in the head and jugular vein or the anterior fontanelle vein. For adults, choose the median cubital vein, the back of the hand, the wrist joint, etc. without congestion and edema, and the vein in individual patients is on the back of the elbow joint. Patients in outpatient clinics take more sitting positions, and patients in wards take more recumbent positions. When taking blood, instruct the patient to relax and keep the environment warm to prevent venous contracture. The restraint time should not be too long, and it is forbidden to beat the arm, otherwise, it may cause local blood concentration or activate the coagulation system. Try to choose a thick and easy-to-fix blood vessel for puncture to ensure that the needle hits the blood. The angle of needle insertion is generally 20-30°. After seeing the blood return, move forward slightly in parallel, and then put on the vacuum tube. The blood pressure of individual patients is low. After the puncture, there is no return blood, but after the negative pressure tube is put on, the blood flows out.

3. Strictly check the validity period of blood collection tubes

It must be used within the validity period, and must not be used when there is foreign matter or sediment in the blood collection tube.

4. Paste the barcode correctly

Print the barcode as directed by the doctor, and paste it on the front after checking, and the barcode cannot cover the scale of the blood collection tube.

5. Timely inspection

Blood samples are required to be sent for inspection within 2 hours after collection to minimize the influencing factors. When submitting for inspection, avoid strong light exposure, shelter from wind and rain, anti-freeze, anti-high temperature, anti-shake, and anti-hemolysis.

6. Storage temperature

The storage environment temperature of blood collection tubes is 4-25°C. If the storage temperature is 0°C or lower than 0°C, it may cause the rupture of blood collection tubes.

7. Protective Latex Cover

The latex cover at the end of the puncture needle can prevent the blood collection test tube from continuing to bleed and pollute the surrounding area and plays the role of sealing blood collection to prevent environmental pollution. The latex cover must not be removed. When collecting blood samples from multiple tubes, the rubber of the blood collection needle may be damaged.