Pipette tips, are you sure you picked the right one?

Pipette operation runs through almost all experimental processes, and the wrong pipette will directly affect the experimental results and even lead to the failure of the experiment.

Not only will they spend extra time and money, but they will also waste the samples they have worked so hard to accumulate, and miss the opportunity to publish the research results first.

Is it simply a matter of maintaining, maintaining and calibrating the pipette regularly to maintain its optimum performance and using the correct pipette technique?

The above process is necessary to ensure accurate pipette results, but other than that, the suction head, a disposable consumable, is the most easily overlooked.It should be known that the suction head is in direct contact with the liquid sample, which will not only affect the accuracy of the pipette results, but also affect the analysis results directly due to the dissolution and the presence of foreign biological pollutants.

Here are a few Tips to keep in mind when choosing a quality suction head:

1.The suction head with poor raw materials, non-100% pure polypropylene, impurities (trace metals, bisphenol A, etc.), additives (plasticizer, release agent, etc.), not only transparent shining suction head, inelastic wall thickness (affecting the seal), but also the dissolved substances affect the experimental results;

2.The size of the suction head with poor manufacturing process is inconsistent, resulting in poor sealing consistency, especially for the multi-channel pipette, which will lead to inconsistent liquid level and affect the accuracy;

3.Poor quality suction head, internal smooth, flow mark, tip notch/burr, will lead to large liquid residue, fluid discharge is not accurate;

Unclean suction head: Biological pollutants such as foreign DNA(DNase)/RNA(RNase)/ pyrogen /ATP/PCR inhibitors/protein (protease) will be introduced in the experiment to affect the relevant experiments.

So what are the characteristics of a perfect pipette suction?

1.Matching with pipette — easy to load and take, ensure sealing;

2.No defects — the shape and surface of the suction head are free from defects or defects, ensuring the pipette accuracy;

3.Pure raw material without additives — avoid the influence of dissolved substances on the experimental results;

4.Clean without biological contamination – The suction head should be protected from the threat of biological contamination and manufactured and packaged in an ultra-clean environment.

5.Compliance with quality standards – The head from a reputable manufacturer will have a certificate attached to the package certifying which tests have verified the product and how to verify that any potential contamination is below a certain detection limit.