What are the classifications of nucleic acid swab preservation solutions?

Nucleic acid detection, That is after collecting human secretions, under laboratory conditions, by analyzing the RNA gene sequence of the virus, the PCR amplification method is used for detection, and clinical etiological diagnosis is carried out? At present, nucleic acid detection is the most effective detection method for early determination of whether a patient is infected with the virus, early detection, and early treatment.

Nucleic acid test swabs are divided into two types: nasal swabs and throat swabs. During the sampling process, the doctor will use a swab like a cotton swab to wipe the throat and tonsils, or the secretions around the nasal cavity. One swipe”, quick and painless. Medical institutions will disinfect the testing area, and medical staff will also disinfect their hands to avoid cross-infection. In general, the sampling process is safe and clean, and there is no need to worry about the risk of infection. 

So what are the types of swab preservation solutions after sampling? There are two types of swab preservation solutions: inactivated and non-inactivated. In the beginning, almost all of the swab preservation solutions used in the market were to directly store live viruses, that is, non-inactivated viruses. Inactivated swab preservation solution, this way of preservation will lead to a high risk of infection for medical staff in sampling, transportation, and testing. In view of this situation, the front line of anti-epidemic needs swab preservation that can directly inactivate the virus. However, it should be noted that while inactivating the virus, it should also consider whether the preservation solution can stably preserve the integrity of viral nucleic acid, so as to avoid the degradation of nucleic acid in the sample before detection, resulting in “false negative” nucleic acid detection, Desheng The production and development of the inactivated virus preservation solution avoids this problem.

To put it simply, nucleic acid detection swab preservation solution can be divided into two categories. Inactivated preservation solution developed by Rollmed is a swab preservation solution with cleavage function, which contains cleavage salt of the inactivated virus to protect nucleic acid by cleavage of protein, and virus preservation and cleavage can be completed in one step. The other is a non-inactivated preservation solution, which, contrary to inactivated preservation solution, can protect the integrity of virus protein and nucleic acid. Besides nucleic acid detection, it can also be used for research on virus culture. The operating environment requirements of the two preservation solutions are as follows.