What is the difference between a cell culture plate and an ELISA plate?

Cell culture plates are mainly used for culturing cells, and their culture methods can be divided into suspension culture and wall culture. And the enzyme labeling plate is mainly used for protein detection after ELISA reaction. So, what is the difference between cell culture plates and enzyme labeling plates?

The material of the cell culture plate is general polystyrene, which is the same as the material of the enzyme labeling plate, but compared with the enzyme labeling plate, there is a fundamental difference. Since some cell cultures need to be walled, the plates need to be surface treated. Otherwise, the walled cells cannot grow on the plates. The difference between culture plates used for culturing bacteria or suspension cells and non-detachable enzyme labeling plates is very small, and many domestic manufacturers use general bacteria culture plates as non-detachable enzyme labeling plates. So what is the difference between them? It is actually very simple. Culture plates usually come with a cover, while non-detachable enzyme labeling plates usually come without a cover.

Enzyme labeling plates can be divided into 48-well plates and 96-well plates according to the number of wells. Generally, 96-well enzyme plates are commonly used, which should be selected according to your enzyme marker. In addition, the enzyme labeling plate is divided into a detachable enzyme labeling plate and non-detachable enzyme labeling plate, for non-detachable enzyme labeling plate, is a whole plate on the slats are connected together, all the holes are connected together; and detachable enzyme labeling plate is the plate above the slats are separated, divided into 12-well slats and 8-well slats, the enzyme labeling plate frame is reusable. If you bought some detachable enzyme plates before, you can buy some enzyme plates only afterward. Different manufacturers make the overall appearance of the enzyme labeling plate is similar, but some small details will be done differently, such as structure, etc. This is mainly because of to use of different enzyme labeling instruments, so when you choose to buy the enzyme labeling plate also consider what your enzyme labeling instrument is like. However, they are generally adaptable, only individual ones will be different.

In addition, there is another problem, because the material of enzyme labeling plate is general polystyrene (PS), and the chemical stability of polystyrene is poor, can be dissolved by a variety of organic solvents, will be strong acid and strong alkali corrosion, not resistant to grease, easy to change color after exposure to ultraviolet light, so in the process of use must pay attention to these.